Diagnostic Findings [Last Modified: ]
Cryptosporidiosis
[Cryptosporidium spp.]
Causal Agent Life Cycle Geographic Distribution Clinical Features Laboratory Diagnosis Treatment

Molecular diagnosis

Agarose gel - PCR for Cryptosporidium
A

A: Agarose gel (2%) analysis of a PCR diagnostic test for detection of Cryptosporidium parvum DNA.  PCR was performed using primers CPBDIAGF and CPBDIAGR.1

  • Lane S: Molecular base pair standard (100-bp ladder).  Black arrows show the size of standard bands.
  • Lane 1: C. parvum positive fecal specimen.  The red arrow shows the diagnostic band of 435 bp for zoonotic Cryptosporidium parvum.

Reference:

Johnson DW, Pieniazek NJ, Griffin DW, Misener L, Rose JB. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl Environ Microbiol 1995;61:3849-55.
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