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[Leishmania spp.]

A	B

A, B: Leishmania tropica amastigotes from an impression smear of a biopsy specimen from a skin lesion.  In A, an intact macrophage is practically filled with amastigotes (arrows), several of which have a clearly visible nucleus and kinetoplast; in B, amastigotes are being freed from a rupturing macrophage.  Patient had traveled to Egypt, Africa, and the Middle East.  Based on culture in NNN medium, followed by isoenzyme analysis, the species was L. tropica.