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Correct Answer

The correct answer is A, microsporidiosis.  This technique stains the microsporidian spore and the spore wall a bright pinkish red.  Often, a belt-like stripe, which also stains pinkish red, is seen in the middle of the spore.  Modified Kinyoun's acid-fast technique is the staining of choice for diagnosis of cryptosporidiosis and cyclosporiasis.  Amebiasis is commonly diagnosed by identification of the ameba cysts in unstained or stained (e.g., iodine) wet mounts preparation or in permanent preparations stained with trichrome.

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