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Correct Answer

The correct answer is B, Gram-chromotrope stain, also know as quick-hot Gram-chromotrope stain.  This is a faster technique compared to the chromotrope 2R (also knows as Weber's chromotrope stain), which combines the chromotrope stain with a step from the Gram's staining procedure.  Use of this procedure results in crisp violet staining of the spores.

Click here to open a new window with images of microsporidian spores stained with Gram-chromotrope stain.  Click here to open a new window with the Gram-chromotrope staining procedure.  Close the new window to return to this page.

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