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The correct
answer is D. Cyclospora cayetanensis is variably acid-fast,
and often absorbs/retains little or no stain. This is the reason that
hot safranin staining and UV fluorescence microscopy have been recommended
as alternative methods to diagnose cyclosporiasis. The other images
seen are Cryptosporidium parvum oocysts (answer A), an Isospora
belli oocyst (answer B), and an atypical artifact (answer C).
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