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The correct
answer is C, collect specimens in 10% formalin; perform a FEA, modified
acid-fast stain, and chromotrope 2R (Weber) stain; examine the acid-fast
slide and the chromotrope 2R slide. PVA fixative is not necessary
for these two parasites and cannot be used to examine either Cryptosporidium
or microsporidia. The FEA done on the formalin specimen would be used
to prepare the acid-fast slide to be examined for Cryptosporidium
and may be used to prepare the chromotrope 2R slide to be examined for microsporidia.
FEA is optional for microsporidia; concentration neither increases nor decreases
these parasites, but it will reduce background. The added advantage
of having FEA material is that a direct fluorescent antibody assay can also
be performed if desired.
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