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Question 10

During a large outbreak of diarrhea in local childcare centers, your laboratory quickly screened a large number of stool specimens for Cryptosporidium sp. and Giardia intestinalis so that public health officials could determine the possible cause of the outbreak.  This task was accomplished by screening the specimens using enzyme immunoassays (EIA).  Almost 30% of the specimens were positive for Cryptosporidium sp.  What is the preferable step to take after obtaining these results?

    (a) Inform the epidemiologists and public health officials of the results so they can investigate the outbreak
    (b) Repeat the EIA and report the positive results if both runs agree
    (c) Confirm results using other techniques such as direct fluorescent antibody assay (DFA) or modified Kinyoun's acid-fast stain
    (d) Collect additional specimens from the patients and repeat the EIA

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