|
| |
Unstained |
Temporary Stains |
Permanent Stains |
| Characteristic |
Saline |
Formalin |
Iodine (Cysts) |
Buffered Methylene Blue (Trophs)1 |
|
| Trophozoites |
| Motility |
+ |
|
|
|
|
| Cytoplasm |
|
|
|
|
|
|
Appearance
|
+ |
+ |
|
+ |
+ |
|
Inclusions
(rbc., bacteria)
|
+ |
+ |
|
+ |
+ |
| Nucleus |
|
+2 |
|
+ |
+ |
| Cysts |
| Nuclei |
|
+
|
+
|
|
+
|
| Chromatoid bodies |
+
|
+
|
+3
|
|
+
|
| Glycogen |
|
|
+
|
|
(vacuole present)
|
1Quensel's stain may be substituted
for buffered methylene blue.
2Nuclei of trophozoites are visible in
formalin-fixed material but are usually not sufficiently
distinctive for species identification.
3Chromatoid bodies are more easily seen in
unstained wet mounts than in iodine preparations.
|
|