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Table 2
Characteristics of Intestinal Flagellates, Ciliate, and Coccidia Visible
in Different Types of Fecal Preparations
| Unstained | Temporary Stains | Permanent Stains | |||
| Characteristic | Saline | Formalin | Iodine (Cysts) |
Neutral Red 1 (Trophs) |
|
| Flagellates | |||||
| Trophozoites | |||||
| Motility | + | | + | | |
| Shape | + | + | + | + (may be distorted) |
|
| Nucleus | | + | + | + | |
| Flagella | ± | | + | ± | |
| Other features2 | + | + | + | + | |
| Cysts | |||||
| Shape | + | + | + | + | |
| Nuclei | | + | + | + | |
| Fibrils | ± | + | + | + | |
| Ciliate (Balantidium coli) | |||||
| Trophozoites | |||||
| Motility | + | | + | | |
| Macronucleus | + | + | + | + | |
| Cilia | + | + | + | + | |
| Cysts | |||||
| Macronucleus | + | + | ± | + | |
| Coccidia | |||||
| Oocysts/Sporocysts | + | + | + | ±3 | |
1Neutral red dye in methocel solutions.
2The undulating membrane of Trichomonas and the spiral groove of Chilomastix may not
be visible in all cases.
3Cryptosporidium oocysts can be demonstrated
in acid-fast stains.
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