The correct answer is C, either electron microscopy or polymerase chain reaction (PCR).  Using electron microscopy, it is possible to visualize features that differentiate microsporidian species, e.g., the number of coils of the polar tubules.  PCR has been successfully used for the detection of Enterocytozoon bieneusi, Encephalitozoon intestinalis, Encephalitozoon hellem, and Encephalitozoon cuniculi in research laboratories.

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